The invention disclose 9-N-substituted berberine derivates, a method for making same and applications as anticancer drugs, wherein the chemical formula is shown as the formula I, n is selected from 1-6; R is selected form H, amino, C1-C6 amido, hydroxyl, alkoxyl, alkoxyl, benzene ring or substituted benzene ring; m is selected from 0-1; anion X<-> is selected from halogen anion, sulfate anion, hydrogen sulfate anion, etc. the 9-N-substituted berberine derivates have strong interaction with protooncogene c-mycDNA rich in guanine, have good inhibitory activity to telomere and telomerase in cancer cells, and have strong inhibition to the expression of protooncogene c-myc. Proved by experiment, the 9-N-substituted berberine derivates of the invention have substantial inhibition to various cancer cell line, have small toxicity to normal cells, and may be used for preparing anticancer drugs. The 9-N-substituted berberine derivates of the invention has a simple preparation method, a substantial potency, and have great medicine market outlook.