The invention discloses an immunity detection chip of prawn white spot syndrome virus (WSSV), comprising a chip carrier and an agarose gel layer which is paved on the chip carrier, wherein, the agarose gel layer is fixed with a plurality of antibody microarrays of 4*4, and chip-dedicated fences or Super PAP Pen scribings are used for separating the microarrays from each other. The invention comprises the following steps: adopting the sandwich method to detect the antigen; fixing the pathogenic polyclonal antibody (PcAb) on the chip slice base; taking the target organ tissue of the individual to be detected to prepare to-be-detected sample liquid; incubating directly the to-be-detected sample liquid with the chip which is fixed with PcAb to capture the antigen and lead the antigen to combine on the chip; adding a specific monoclonal antibody probe marked by fluorescence; and reading results through a CCD chip scanner. The invention has the advantage of detecting white spot syndrome virus (WSSV) in multiple samples simultaneously, and is applicable to the rapid and accurate detection of white spot syndrome virus (WSSV) of prawns/crabs in breeding production and the quarantine inspection of WSSV in import and export prawns/crabs.