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发明授权
CN101792766B 一种生产猪生长激素的方法及其专用DNA片段 有权

一种生产猪生长激素的方法及其专用DNA片段
摘要：
本发明公开了一种生产猪生长激素的方法及其专用DNA片段。该DNA片段，是如下1)或2)：1)其核苷酸序列如序列表中序列3的自5’端6-1108位所示；2)其核苷酸序列如序列表中序列3所示。含有上述DNA片段的重组载体也属于本发明的保护范围之内。具体地，上述重组载体是将上述的DNA片段插入pcDNA3.1(+)的多克隆位点构成的重组表达载体。含有上述DNA片段的转基因细胞系也属于本发明的保护范围之内。上述转基因细胞培养后，pcDNA-GH12组GH表达量是转pcDNA质粒对照组的16410倍；pcDNA-GH12组的GH与内参beta-actin比值为0.278，显著高于正常对照组(转pcDNA空载体组)的0.016。说明含有第1，2内含子的情况下，GH的表达量更高。
摘要（英）：

The invention discloses a method for producing porcine growth hormones and a special DNA fragment thereof. The DNA fragment is shown in (1) or (2), wherein a nucleotide sequence of the DNA fragment (1) is shown from the 5' end 6-1108 site of the sequence 3 in a sequence table, and the nucleotide sequence of the DNA fragment (2) is shown in the sequence 3 in the sequence table. A recombinant vector containing the DNA fragment also belongs to the scope of protection of the invention. Particularly, the recombinant vector is a recombinant expression vector formed by inserting the DNA fragment into multiple cloning sites of pcDNA3.1 (+). A transgenic cell line containing the DNA fragment also belongs to the scope of protection of the invention. After the transgenic cells are cultured, the expression quantity of GH of the pcDNA-GH12 group is 16410 times of the expression quantity of GH of a transgenic pcDNA plasmid control group, and the ratio of GH to beta-actin of the pcDNA-GH12 group is 0.278 which is obviously higher than 0.016 of the normal control group (transgenic pcDNA empty vector group). The result shows that under the condition of existence of 1,2 intron, the expression quantity of the GH is higher.

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