The invention relates to a fusion protein with high-efficient platelet-increasing role, and the fusion protein consists of two TPO mimetic peptides which are connected in series and a human growth hormone molecule. The fusion protein can significantly improve the bioavailability in the aspect of platelet generation, reduce the using quantity and reduce the toxicity or the side effects. The fusionprotein can greatly reduce the treatment cycle and the treatment time, and the platelet-increasing effect of the fusion protein is more significant. The fusion protein can be used for producing drug preparations to treat a variety of primary or secondary thrombocytopenia and other diseases.