The invention provides a curvularia lunata strain and application thereof. The strain is curvularia lunata B6 of which the preservation number is CGMCC No.2928. The curvularia lunata strain B6 CGMCC No.2928 has strong and fast pathogenicity to echinochloa crusgalli; field experiments show that the echinochloa crusgalli shows spot and dead symptoms in a week after spores of the curvularia lunata strain are sprinkled to the echinochloa crusgalli and the fresh weight inhibition rate to the echinochloa crusgalli can reach 90 percent in 28 days. The fungus strain curvularia lunata strain B6 CGMCC No.2928 is separated from echinochloa crusgallis naturally having diseases in fields without any artificial gene modification; therefore, the curvularia lunata strain cannot have any ecological and environmental hidden danger when released to nature and is an absolutely-safe biological prevention and control medium.