利索-全球专利检索

  1. 登录
  2. 注册
发明公开
CN101979501A 表达猪链球菌2型表面抗原基因sao的重组猪霍乱沙门氏菌及疫苗与应用 有权

表达猪链球菌2型表面抗原基因sao的重组猪霍乱沙门氏菌及疫苗与应用
摘要：
本发明属于动物细菌基因工程领域，具体涉及一种不含抗性标记的表达猪链球菌2型表面抗原sao基因片段的重组猪霍乱沙门氏菌株的构建、疫苗制备及应用。获得一株无抗性标记的表达猪链球菌2型sao基因片段的重组猪霍乱沙门氏菌asd-C500/Pya-saoA，其保藏号为CCTCC NO：M2010156。该重组菌缺失了猪霍乱沙门氏菌的asd基因，含有能在该菌株中表达asd和猪链球菌2型sao基因片段的质粒pYA-saoA。本发明还公开了该重组菌株与疫苗的制备方法以及在制备猪霍乱沙门氏菌-猪链球菌2型疫苗中的应用。
摘要（英）：

The invention belongs to the field of animal bacterium gene engineering, and in particular relates to construction of resistance marker-free recombinant Salmonella choleraesuis for expressing surface antigen sao gene segment of streptococcus suis type 2, preparation of a vaccine and application. The resistance marker-free recombinant Salmonella choleraesuis for expressing the surface antigen sao gene segment of the streptococcus suis type 2, namely asd-C500/Pya-saoA is obtained, and the collection number is CCTCC NO: M2010156. The asd gene of the Salmonella choleraesuis is deleted in the recombinant strain, and the recombinant strain contains plasmid pYA-saoA capable of expressing the asd and the sao gene segment of the streptococcus suis type 2. The invention also discloses a method for preparing the recombinant strain and the vaccine, and application in preparing Salmonella choleraesuis-streptococcus suis type 2 vaccines.

公开/授权文献
0/0