The invention relates to a 3-ketone-6-substituted-benzofuran compound as well as a preparation method and application thereof. The 3-ketone-6-substituted-benzofuran compound has the following structural formula: the preferable 3-ketone-6-substituted-benzofuran compound can be used for preparing medicament capable of inhibiting the biological activity of the microorganism. Compared with the prior art, the 3-ketone-6-substituted-benzofuran compound takes 3-ketone substituent group benzofuran as a aromatic ring center; (5-methyl-2-phenyloxazole-4-yl) ethyoxyl is introduced into the 6th position of the benzofuran to obtain a novel compound; the preparation method of the compound is built and optimized; the prepared novel compound is subjected to a bacteriostat screening experiment; a primary in-vitro bacteriostat experiment confirms that the parts of prepared new compounds have excellent broad spectrum bacteriostasis activity; and the 3-ketone-6-substituted-benzofuran compound can be used for preparing the novel antibacterial medicament.