The invention discloses a spacing and locking device for an automobile rear spare wheel frame, and the device comprises a clamping lever mechanism, an inhaul cable mechanism and a spacing and locking mechanism, wherein the spacing and locking mechanism is fixedly connected with a wheel frame and an automobile body; and the inhaul cable mechanism is respectively connected with the clamping lever mechanism and the spacing and locking mechanism. According to the spacing and locking device for the automobile rear spare wheel frame, the spacing force of the rear spare wheel frame can be increased to realize firm spacing and locking. The existing three-point spacing structure is optimized into a two-point spacing structure, a rear back door is liberated, the working condition of the rear back door is improved, the service life is prolonged, and the risk of abnormal sound is eliminated. The spacing and locking device for the automobile rear spare wheel frame is suitable for serving as the spacing and locking device for the automobile rear spare wheel frames of various automobile types.