The invention discloses a method for detecting live microorganisms in vacuum-packed cooked meat products after ultrahigh pressure treatment. The method is characterized in that after the vacuum-packed cooked meat products are subjected to ultrahigh-pressure non-heat sterilization treatment, the live microorganisms in the vacuum-packed cooked meat products effectively grow and are accurately counted after being cultivated through a two-layer selective culture medium; a method for preparing the two-layer selective culture medium comprises the steps of pouring a lactic acid bacterium culture medium into a sterile culture dish at first, after the lactic acid bacterium culture medium is cooled and solidified, pouring a flat-plate counting agar culture layer to the surface of the lactic acid bacterium culture medium, controlling a thin-layer flat-plate counting agar culture layer to be paved on the surface of the lactic acid bacterium culture medium, and then putting the culture medium under an ultraviolet lamp for solidification. The major design key point of the method disclosed by the invention is the preparation of the two-layer selective culture medium; the culture medium is mainly used for hygienic inspection of microorganisms of vacuum-packed cooked meat products after ultrahigh pressure sterilization treatment; and as a result, the problem that a common culture method is difficult to detect the live microorganisms of the vacuum-packed cooked meat products after ultrahigh pressure treatment and even incapable of detecting the live microorganisms is solved.