利索-全球专利检索

  1. 登录
  2. 注册
发明公开
CN103374558A 一种一步法裂解CPC生产7-ACA的酰化酶及编码它的多核苷酸 失效 - 权利终止

一种一步法裂解CPC生产7-ACA的酰化酶及编码它的多核苷酸
摘要：
本发明公开了一种一步法裂解CPC生产7-ACA的酰化酶及编码它的多核苷酸，含有该核苷酸序列的重组表达载体和重组表达转化体及其制备方法，以及该重组酶在合成7-ACA中的应用。所述CPC酰化酶是如下(a)或(b)的蛋白质：(a)SEQ ID NO.3所示的氨基酸序列的第180位的缬氨酸经过取代且具有CPC酰化酶活性的蛋白质；(b)在(a)中的氨基酸序列的N端或C端添加一个或几个氨基酸且具有CPC酰化酶活性的由(a)衍生的蛋白质。本发明公开的CPC酰化酶的酶活性和底物耐受性均有提高，从而为CPC酰化酶的人工定向进化改造提供了有力前提。
摘要（英）：

The invention discloses an acylase for producing 7-ACA (Aminocephalosporanic acid) by splitting CPC (Cephalosporin C) by utilizing a one-step method, polynucleotide encoding the same, a recombinant expression vector containing a nucleotide sequence, a recombinant expression transformant containing the nucleotide sequence, a preparation method of the acylase and an application of a recombinase in synthesis of the 7-ACA. A CPC acylase is a protein of (a) or (b) as follows: (a) a protein which has CPC acylase activity and an amino acid sequence represented by SEQ ID NO.3, wherein 180-site valine is substituted; and (b) a protein which has the CPC acylase activity and an amino acid sequence formed by adding one or multiple amino acids to an N terminal or a C terminal of the amino acid sequence in the (a) and is derivative from (a). According to the CPC acylase disclosed by the invention, both the enzyme activity and the substrate tolerance are improved, so that a strong precondition is provided for manual determinated evolution transformation of the CPC acylase.

公开/授权文献
0/0