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发明公开
CN103642777A 一种提高米曲霉木聚糖酶热稳定性的方法 无效 - 撤回

一种提高米曲霉木聚糖酶热稳定性的方法
摘要：
本发明提出了一种新型的利用计算机技术与生物信息学方法对常温的木聚糖酶进行定向改造的方法，运用基因工程技术构建出了具有高效表达能力的突变木聚糖酶工程菌。将来源于米曲霉(Aspergillus oryzae)CICC40186菌株的常温木聚糖酶进行热稳定性的定向改造，获得了一种木聚糖酶突变体，其热稳定性有了一定的提高，而其催化活性较原酶无明显改变。作为一种耐热型的酶制剂，该木聚糖酶具有较大的工业化生产潜力和应用价值。
摘要（英）：

The invention discloses a novel method for carrying out directed modification on normal-temperature xylanase by utilizing a computer technology and a bioinformatics method, which constructs mutant xylanase engineering bacteria with efficient expression ability by applying a genetic engineering technology. A xylanase mutant is obtained by carrying out directed modification of thermal stability on the normal-temperature xylanase from an aspergillus oryzae CICC40186 bacterial strain, wherein thermal stability of the xylanase mutant is improved to a certain extent, and catalytic activity of the xylanase mutant is not changed obviously in comparison with proenzyme. As a heat-resisting enzyme preparation, the xylanase has great industrial production potential and economic value.

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