The invention provides a compound starch biochemical anti-aging agent and a method of applying the same for improving cake quality, and belongs to the technical field of food processing. The compound starch biochemical anti-aging agent comprises low-temperature fungal alpha amylase, molecular distillation monoglyceride and sodium stearoyl lactylate. The method includes evenly mixing the low-temperature fungal alpha amylase, molecular distillation monoglyceride and sodium stearoyl lactylate wit filtered flour, slowly adding egg paste and mixing, performing injection molding, baking instantly, cooling to the room temperature and packing to obtain finished products. By means of controlling of wheat starch transformation under the affect of the low-temperature fungal alpha amylase, limited enzymatic hydrolysis of starch in the cake producing process is implemented, the anti-retrogradation effect of starch of compound emulsifier is combined, the rate of starch retrogradation is reduced greatly, shelf lives of cakes are prolonged, the quality of the cakes is improved, the products has small, even and fine pores, taste is soft, delicate and sweet, and water loss rate is reduced.