The invention discloses an industrial manufacturing method and a use of an allergen-eliminated partly appropriately-hydrolyzed casein peptide, and belongs to the technical field of food nutrition and biotechnology. The industrial manufacturing method utilizes food-grade casein as a raw material and realizes preparation of the appropriately-hydrolyzed casein peptide powder by a composite enzymatic hydrolysis-ultrafiltration combined technology. The allergen-eliminated partly appropriately-hydrolyzed casein peptide has average molecular weight of 1500Da-3000Da, protein antigenicity reduced by more than 85%, and has main allergen alpha s1-casein content reduced by more than 95% than casein content. Compared with other appropriately-hydrolyzed casein products on the market, the allergen-eliminated partly appropriately-hydrolyzed casein peptide has obviously reduced sensitization activity and has a small bitter taste and good processing performances. The allergen-eliminated partly appropriately-hydrolyzed casein peptide prepared by the industrial manufacturing method can be used as a novel functional nutrient in common foods, sports functional foods, infant formula milk powder and foods, adult milk powder and foods, milk powder and foods for middle and old age, health foods and medicines and has a wide market prospect.