The invention discloses a method for high-flux identification of ralstonia solanacearum resistance. The method comprises the following steps: sterilizing quartz sand with particle size ranging from 2 mm to 4 mm at high temperature of 121 DEG C for 30 minutes, cooling, and spreading into an aseptic 12-pore cell culture plate, wherein each pore is filled with 10 g of the quartz sand and 4 ml of aseptic water; sowing flue-cured tobacco seeds to be tested onto the quartz sand of the 12-pore cell culture plate, after sowing, and culturing by the 12-pore cell culture plate under the conditions of 25 DEG C, RH of more than 80% and alternative 16-hour lighting and 8-hour shading; after the flue-cured tobacco seeds germinate, adding a Hoagland's nutrient solution into the 12-pore cell culture plate for culturing the flue-cured tobacco seeds under the aseptic condition, continuously culturing under the conditions of 25 DEG C, RH of more than 80% and alternative 16-hour lighting and 8-hour shading, and identifying the disease resistance of the flue-cured tobacco seeds each with 5-6 leaves, wherein each pore is filled with 2 ml of the Hoagland's nutrient solution; preparing inoculating liquid and inoculating; investigating and grading the disease states. According to the method, the ralstonia solanacearum resistance can be identified with high flux and high efficiency and a large amount of flue-cured tobacco varieties can be identified in one time.