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发明公开
CN104327188A 一种美国白蛾SUMO-ABP-dHC-CecropinA融合蛋白及其应用 失效 - 权利终止

一种美国白蛾SUMO-ABP-dHC-CecropinA融合蛋白及其应用
摘要：
本发明公开了一种美国白蛾SUMO-ABP-dHC-CecropinA融合蛋白及其应用，该美国白蛾SUMO-ABP-dHC-CecropinA融合蛋白表达方法如下：构建pETSUMO-ABP-dHC-CecropinA表达载体，在大肠杆菌中表达，超声破碎后收集上清Ni+亲和纯化，梯度洗脱之后得到美国白蛾SUMO-ABP-dHC-CecropinA融合蛋白。本发明通过基因重组方法获得美国白蛾SUMO-ABP-dHC-CecropinA融合蛋白，并在大肠杆菌中获得高效表达，目的多肽经过HPLC分析达到大95%的纯度，通过质谱分析，分子量与预测多肽分子量也吻合。经检测，多肽具有明显的抗真菌活性，所制备的目的多肽，其活性高、成本低。
摘要（英）：

The invention discloses fall webworm SUMO-ABP-dHC-Cecropin A fusion protein and an application thereof. An expression method of the fall webworm SUMO-ABP-dHC-Cecropin A fusion protein comprises the following steps: establishing a pETSUMO-ABP-dHC-Cecropin A expression carrier, expressing in escherichia coli, performing ultrasonic crushing, collecting supernate Ni<+>, performing affinity purification, and performing gradient elution, thereby obtaining the fall webworm SUMO-ABP-dHC-Cecropin A fusion protein. The fall webworm SUMO-ABP-dHC-Cecropin A fusion protein is obtained by using a genetic recombinant method and can be efficiently expressed in escherichia coli, after HPLC analysis, target polypeptide can achieve the purity greater than 95%, and through mass spectrometry, the molecular weight fits for prediction polypeptide molecular weight. The detection shows that the polypeptide has remarkable antifungal activity, and the prepared polypeptide is high in activity and low in cost.

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