A signal collection and power connection assembly of a power battery module, a power battery module and a vehicle are provided. The assembly includes a substrate, a power connection member fixed on the substrate, a power connection line formed by a first sheet-like conductor disposed on the substrate, a signal collection line formed by a second sheet-like conductor disposed on the substrate, and a signal collection member disposed on the substrate and coupled with the signal collection line. A terminal of the power connection line is coupled with the power connection member, and a terminal of the signal collection line is coupled with the power connection member.