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CN104388451A 耐热木聚糖酶(Xyl11M)基因的合成及其在毕赤酵母中的表达 无效 - 撤回

  • 专利标题： 耐热木聚糖酶(Xyl11M)基因的合成及其在毕赤酵母中的表达
  • 专利标题（英）： Synthesis of thermostable xylanase gene (Xyl11M) and expression of gene in pichia pastoris
  • 申请号： CN201410758941.1
    申请日： 2014-12-10
  • 公开(公告)号： CN104388451A
    公开(公告)日： 2015-03-04
  • 发明人: 邬敏辰 , 姚瑶 , 吴芹 , 李剑芳 , 李兴倩 , 张鹏
  • 申请人： 江南大学
  • 申请人地址： 江苏省无锡市滨湖区蠡湖大道1800号
  • 专利权人： 江南大学
  • 当前专利权人： 江南大学
  • 当前专利权人地址： 江苏省无锡市滨湖区蠡湖大道1800号
  • 主分类号： C12N15/56
  • IPC分类号： C12N15/56 ;  C12N9/42 ;  C12N15/81 ;  C12R1/84
耐热木聚糖酶(Xyl11M)基因的合成及其在毕赤酵母中的表达
摘要：
本发明为耐热木聚糖酶(Xyl11M)基因的合成及其在毕赤酵母中的表达，涉及一种来源于嗜热裂孢菌(Thermobifida fusca)的耐热β-1,4-内切木聚糖酶(Xyl11M)在毕赤酵母中表达、活性测定及酶学性质分析方法。其基因命名为xyl11M，它的核苷酸序列为SEQ ID NO:1，编码的蛋白质为Xyl11M，氨基酸序列为SEQ ID NO:2。所制备的重组酶的活性为157U/mL；其最适反应温度为70℃，70℃下保温60min，残余酶活为98％，表明该酶热稳定性非常好；其最适pH为6.0，在pH 5.0～8.0范围内较稳定；金属离子对该酶活性影响较小。作为一种耐热型的酶制剂，该酶具有较大的工业化应用潜力及经济价值。
摘要（英）：

The invention discloses synthesis of a thermostable xylanase gene (Xyl11M) and expression of the gene in pichia pastoris, and relates to a method for expression, activity determination and enzymatic property analysis of the thermostable beta-1 4-endoxylanase gene (Xyl11M) in the pichia pastoris. The thermostable xylanase gene is named as Xyl11M and has a nucleotide sequence of SEQ ID NO:1 and an amino acid sequence of SEQ ID NO:2, and coded protein is Xyl11M; the activity of prepared recombinase is 157 U/mL; the optimal reaction temperature is 70 DEG C and is kept for 60 minutes, and the activity of residual enzyme is 98% which shows that the thermostability of the enzyme is very good; the optimal pH value is 6.0 and the enzyme is relatively stable when the pH value is 5.0-8.0; the influence of metal ions on the enzyme activity is relatively small. As a thermostable enzyme preparation, the xylanase has relatively high industrialized application potentials and economic values.

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