The invention relates to a probiotic fermentation milk drink with the bowel relaxing and blood fat reduction assisting functions and a making method thereof. The method comprises the steps that on the basis that trypsin is utilized for hydrolyzing reconstituted skimmed milk to obtain skimmed milk enzymatic hydrolysate, whey powder conditioning fluid, beer, banana jam, sweet potato jam, fructo-oligosaccharide and xylitol are added, and hawthorns, roots of red-rooted salvia and a traditional Chinese medicine extract of roots of kudzu vine are added to prepare a mixed nutrient solution; after heat treatment cooling is performed, inoculation of a mixed probiotic leavening agent is performed, and the probiotic fermentation milk drink with the bowel relaxing and blood fat reduction assisting functions is obtained after fermentation is performed for 24-72 h. The probiotic fermentation milk drink with the bowel relaxing and blood fat reduction assisting functions can remarkably lower the TC, TG, LDL-C content in rat serum, remarkably increase the HDL-C and ApoAI content, remarkably lower the ALT and AST levels of the rat serum, remarkably improve the SOD activity of liver tissue and effectively relieve liver injuries caused by lipid peroxidation, and has the good bowel relaxing and blood fat reduction assisting functions.