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发明公开
CN104694537A 诱导表达抗菌肽的植物表达载体及其用途 无效 - 驳回

诱导表达抗菌肽的植物表达载体及其用途
摘要：
本发明属于柑桔品质改良技术领域，具体涉及诱导表达抗菌肽的植物表达载体及其用途。本发明要解决的技术问题是为改良柑桔溃疡病抗性提供一种新选择。本发明的技术方案是诱导表达抗菌肽的表达框架，其特征在于：采用柑桔溃疡病病原菌高效诱导表达启动子调控天蚕素抗菌肽的表达。本发明还提供了还有所述表达框架的植物表达载体以及含有所述植物表达载体的宿主细胞。本发明还提供了增强柑桔溃疡病抗性的方法。本发明通过将溃疡病高效启动子调控下的抗菌肽基因mAATCB整合到柑桔基因组中,实现了抗菌肽基因在柑桔受溃疡病病原菌入侵时迅速高水平表达；从而增强了柑桔溃疡病抗性。
摘要（英）：

The invention belongs to the technical field of citrus quality improvement, and particularly relates to a plant expression vector for antimicrobial peptide induced expression and application thereof. The invention aims to provide a new option for improving citrus canker resistance. The technical scheme is an expression cassette for antimicrobial peptide induced expression. The invention is characterized in that the citrus canker pathogen is adopted to efficiently induce the expression of the expression promoter control cecropin antimicrobial peptide. The invention also relates to a plant expression vector containing the expression cassette and a host cell containing the plant expression vector. The invention also relates to a method for enhancing citrus canker resistance. The antimicrobial peptide gene mAATCB under the control of the canker high-efficiency promoter is integrated into the citrus genome, thereby implementing quick high-level expression of the antimicrobial peptide gene when the citrus is infected by the canker pathogen, and further enhancing the citrus canker resistance.

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