The invention discloses a preparation method of aldehyde flavor derivatives. According to the preparation method, an aldehyde flavor and amino acid methyl ester hydrochloride react in an organic solvent containing base and a water absorbent, wherein the aldehyde flavor is one ingredient of neral, geranial, alpha-cyclocitral, beta-cyclocitral and beta-cyclohomocitral; and molar ratio of the aldehyde flavor to amino acid methyl ester hydrochloride is 0.9-1:1. The prepared aldehyde flavor amino acid derivative and aldehyde flavor amino acid methyl ester derivative have stable chemical properties, are not easy to volatilize, will not generate obvious fragrance before being heated, can make fragrance of cigarettes to be richer, make smoke to be softer and make remaining taste to be richer after being heated, and are beneficial to prolong shelf life of cigarettes after flavoring.