The invention relates to an engine fuel oil manifold assembly noise test method. An anechoic room and an ECU in electrical control connection with a to-be-measured engine fuel oil manifold assembly are included, wherein the ECU is respectively in electrical connection with a signal generator and a storage battery through an engine wire harness, the anechoic room is internally provided with multiple elastic ropes used for hanging the to-be-measured engine fuel oil manifold assembly, the anechoic room is further provided with five acoustic sensors internally, and the five acoustic sensors are respectively positioned at the horizontal front portion, the horizontal back portion, the horizontal left portion, the horizontal right portion and the vertical upper portion of the to-be-measured engine fuel oil manifold assembly. The invention further provides an engine fuel oil manifold assembly noise test system. Through the test method and the test system, oil injection noise can be measured safely, efficiently and precisely.