利索-全球专利检索

  1. 登录
  2. 注册
发明公开
CN105112344A 一株产角蛋白酶的副短短芽孢杆菌及其应用 无效 - 驳回

一株产角蛋白酶的副短短芽孢杆菌及其应用
摘要：
本发明公开一株角蛋白产生菌株及其培养条件和酶学性质，属于微生物技术领域。经鉴定，该菌株为副短短芽孢杆菌(Brevibacillus parabrevis)，命名为副短短芽孢杆菌R-7，现保藏于中国微生物菌种保藏管理委员会普通微生物中心，保藏号为CGMCC No.10798。经初步优化，该菌株的培养基为：羊毛1%，可溶性淀粉1%，玉米粉1%，NaCl 0.15%，NH4Cl 0.05%，K2HPO4 0.03%，KH2PO4 0.04%，MgCl2·6H2O 0.01%。摇床培养条件为：初始pH值为8.0，接种量2%，培养温度40℃，转速220r/min，装液量30mL/250mL，发酵周期16h，产角蛋白酶活力达到412U/mL。该角蛋白酶最适作用温度为40℃，最适pH为7.0。该酶在制革及医药等领域具有重要应用。
摘要（英）：

The invention discloses a keratinase producing strain and culture conditions and enzymatic properties thereof, and belongs to the technical field of microorganisms. As identified, the strain is Brevibacillus parabrevis, is named as Brevibacillus parabrevis R-7 and now is preserved in China General Microbiological Culture Collection Center, with preservation number CGMCC No.10798. After preliminary optimization, the culture medium of the strain comprises 1 percent of wool, 1 percent of soluble starch, 1 percent of corn meal, 0.15 percent of NaCl, 0.05 percent of NH4Cl, 0.03 percent of K2HPO4, 0.04 percent of KH2PO4 and 0.01 percent of MgCl2 6H2O. Shaker culture conditions include initial pH 8.0, inoculation amount 2 percent, culture temperature 40 DEG C, revolving speed 220r/min, liquid volume 30mL/250mL and fermentation period 16h, wherein the activity of the produced keratinase reaches 412U/mL. The optimum acting temperature of the keratinase is 40 DEG C and the optimum pH is 7.0. The enzyme has important application in fields such as tanning and medicines.

0/0