The invention relates to a high-pass multipurpose controllable microorganism inoculation device and a batch strain inoculation method to solve the problems of the prior art that strain screening workload is high, pollution is serious, reagent waste is high and visuality contrast effect is poor. The device comprises an inoculator, a sample tube support, and centrifugal tubes or test tubes arranged in the sample tube support, wherein the inoculator comprises a base plate and an inoculation needle array perpendicularly installed on the base plate, the inoculation needle array is composed of inoculation needles arranged in a certain way, and the number and arrangement mode of the centrifugal tubes or test tubes correspond to those of the inoculation needle array so that the inoculation needles can be sunk into the corresponding centrifugal tubes or test tubes when the inoculator is connected with the sample tube support. The device and method have the advantages that one-time batch cultivation of strains is achieved, structure is simple, implementation is easy, reagents are saved, contrasting can be conducted under the condition that cultivation environments are consistent, and test results are more accurate and reliable.