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发明公开
CN105418755A 速效胰岛素前体蛋白以及速效胰岛素的制备方法 审中-实审 复审申请

速效胰岛素前体蛋白以及速效胰岛素的制备方法
摘要：
本发明提供一种门冬胰岛素前体，其氨基酸序列为：Z1(XY)nZ2Z3Z4-B(1-29)-C1C2C3-A(1-21)，其中Z1、X、Z2分别为Asp或Glu；Y为Ala或Gly；n为1~10之间的整数；Z3为Pro、Glu或Asp；Z4、C3分别为Lys或Arg；C1、C2分别为Glu、Asp、Ala或Gly；B(1-29)为门冬胰岛素B链1-29位氨基酸；并且A(1-21)为门冬胰岛素A链1-21位氨基酸。本发明还提供其编码基因、包含该编码基因的克隆载体或表达载体、转化体，以及利用该速效胰岛素前体蛋白制备速效胰岛素的方法。本发明的产物表达量高的优势、发酵表达产物纯度高、提取方法简单且收率高。
摘要（英）：

The invention provides an insulin aspart precursor. The amino acid sequence is Z1(XY)nZ2Z3Z4-B(1-29)-C1C2C3-A(1-21), wherein Z1, X and Z2 are Asp or Glu respectively, Y is Ala or Gly, n is an integer from 1-10, Z3 is Pro, Glu or Asp, Z4 and C3 are Lys or Arg respectively, C1 and C2 are Glu, Asp, Ala or Gly respectively, B(1-29) is insulin aspart B chain 1-29 bit amino acid, and A(1-21) is insulin aspart A chain 1-21 bit amino acid. The invention also provides a coding gene, a cloning vector or an expression vector containing the coding gene, a transformant, and a method for preparing quick-acting insulin by utilization of the quick-acting insulin aspart precursor protein. The expression level of the product is high, the fermentation expression product purity is high, the extraction method is simple and the yield is high.

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