The invention discloses a racemization recycling method of Pregabalin intermediate mother liquor from resolution. The method comprises the following steps: (1) after resolution of 3-(carbamoymethyl)-5-methylhexanoic acid racemate is carried out by using (R)-phenylethylamine, mother liquor is condensed and dissolved in water, concentrated hydrochloric acid is used for adjusting pH value to 1-3, cooling is carried out for crystallization, and (S)-3-(carbamoymethyl)-5-methylhexanoic acid is obtained; (2) (S)-3-(carbamoymethyl)-5-methylhexanoic acid is added into xylene, after dissolving, temperature is raised to 80-150 DEG C for dehydration and cyclization, and 3-isobutylglutarimid is obtained; (3) under an alkaline condition, 3-isobutylglutarimid is hydrolyzed, and 3-(carbamoymethyl)-5-methylhexanoic acid racemate is obtained. Xylene is selected as a solvent for the dehydration and cyclization reaction, so that recycling yield is high, reaction time is short, expensive reagents are not needed and cost is low; the method is suitable for industrial production.