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发明公开
CN105861416A 一种用于全悬浮培养昆虫细胞的无血清培养基及其制备方法和应用 有权

一种用于全悬浮培养昆虫细胞的无血清培养基及其制备方法和应用
摘要：
本发明提供一种用于全悬浮培养昆虫细胞的无血清培养基，所述培养基由以下组分组成：Grace’s Insect Medium培养基，BFPM401培养基，葡萄糖2?4g/L，水解乳蛋白1?3g/L，PF68 1?3g/L，酵母粉2?5g/L。本发明还提供其制备方法和应用。经试验，本发明无血清培养基能够很好的全悬浮培养昆虫细胞，最大细胞增殖浓度分别为42.5×105/mL（培养Sf?21细胞）和45.4×105/mL（培养Hi?five细胞）。
摘要（英）：

The invention provides a serum-free medium for culturing insect cells in a full suspension mode. The serum-free medium is prepared from a Grace's Insect Medium, a BFPM410 medium, 2-4 g/L of glucose, 1-3 g/L of lactalbumin hydrolysate, 1-3 g/L of PF68 and 2-5 g/L of yeast powder. The invention further provides a preparation method and application of the serum-free medium. As shown in experiments, the serum-free medium can be well used for culturing insect cells in the full suspension mode, the maximum cell proliferation concentration is 42.5*10<5>/mL when the serum-free medium is used for culturing Sf-21 cells, and the maximum cell proliferation concentration is 45.4*10<5>/mL when the serum-free medium is used for culturing Hi-five cells.

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