The invention belongs to the bio-technical field, in particular to a kit for amplifying an influenza virus A whole genome and a using method thereof. The kit comprises a reverse transcription primer mixture, a reverse transcription buffer solution, reverse transcriptase, a PCR amplification buffer solution and DNA polymerase. According to the kit, one reverse transcription primer and a pair of general type amplification primers are designed according to the influenza virus A by adopting two steps of reverse transcription and PCR amplification. The kit can simultaneously amplify eight gene segments of all subtype influenza viruses A, has relatively strong broad spectrum activity and can complete reaction within 6 hours, and an amplification product can be used for molecular cloning, sequencing reaction and the like, so that a simple and quick means is provided for amplifying the influenza virus A whole genome.