The invention provides a limiting and supporting mechanism and a transport vehicle with the limiting and supporting mechanism. The transport vehicle comprises a vehicle frame, fixing blocks arranged on the vehicle frame, a bottom platform arranged on the vehicle frame, a turnover platform and the limiting and supporting mechanism. The limiting and supporting mechanism is arranged on the turnover platform and comprises pin shafts, first limiting blocks, second limiting blocks, turnover assemblies and rotary boards. The two ends of each pin shaft are fixed to the turnover platform. The first limiting blocks are fixed to the pin shafts and each provided with a slope inclining relative to the axis of the corresponding pin shaft. The second limiting blocks are fixed to the pin shafts and spaced from the first limiting blocks. Each turnover assembly comprises a sleeve and a turnover block fixed to the sleeve, and the sleeve is rotationally arranged on the corresponding pin shaft in a sleeving manner and located between the corresponding first limiting block and the corresponding second limiting block. One end of each rotary board is hinged to the turnover platform, and the other end of each rotary board is a free end. By the adoption of the limiting and supporting mechanism, the safety of the bottom platform and the turnover platform of the transport vehicle can be guaranteed, time and labor are saved, and safety and reliability are obtained.