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发明公开
CN106337059A 一种改良柑桔黄龙病抗性的方法 审中-实审

一种改良柑桔黄龙病抗性的方法
摘要：
本发明属于分子生物学技术领域，具体涉及一种改良柑桔黄龙病抗性的方法。本发明要解决的技术问题是为提高柑桔对黄龙病的抗性，提供一种新选择。本发明的技术方案是一种改良柑桔黄龙病抗性的方法，具体如下：将韧皮部特异启动子调控下的抗菌肽基因整合到柑桔基因组中；所述的抗菌肽基因为抗菌肽基因PR1aCB,CB,或PR1aCBer，CB具有如SEQ ID No.1所示的核苷酸序列，R1aCB具有如SEQ ID No.2所示的核苷酸序列，PR1aCBer具有如SEQ ID No.3所示的核苷酸序列。本发明韧皮部特异启动子指导抗菌肽等抗性基因在韧皮部高效表达，进而增强柑桔黄龙病的抗性水平。
摘要（英）：

The invention belongs to the technical field of molecular biology, in particular relates to a method for improving citrus huanglongbing resistance, and aims to solve the technical problem of providing a new choice for improving the citrus huanglongbing resistance. The technical scheme of the invention is the method for improving the citrus huanglongbing resistance. The method is particularly as follows: integrating an antibacterial peptide gene into a citrus genome under the regulation and control of a phloem specific promoter, wherein the antibacterial peptide gene is an antibacterial peptide gene PR1aCB, CB or PR1aCBer; the CB has the nucleotide sequence as shown in SEQ ID No.1; the PR1aCB has the nucleotide sequence as shown in SEQ ID No.2; the PR1aCBer has the nucleotide sequence as shown in SEQ ID No.3. The phloem specific promoter guides resistance genes, such as the antibacterial peptide and the like, to be efficiently expressed in the phloem, so that the citrus huanglongbing resistance level is enhanced.

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