The invention relates to preparation and application of a cotransport system of a Mn<2+> donor and chloroquine drugs. According to the preparation and the application, the problems of low pesticide effect, large required dosage, poor targeting ability and serious toxic and side effects of tumor treating drugs can be effectively solved. According to the technical scheme, double-layer hollow mesoporous manganese sesquioxide nanoparticles are synthesized by virtue of a hydrothermal method, hyaluronic acid and the double-layer hollow mesoporous manganese sesquioxide nanoparticles are linked through chemical bonds so as to spontaneously form a nano-layer in a water medium, and then a chloroquine anti-tumor drug enters mesoporous structures of the double-layer hollow mesoporous manganese sesquioxide nanoparticles through a physical effect. According to the drug-cooperated cotransport system, the dosage and toxic and side effects of the drug can be effectively reduced, the treating efficiency of the drug can be improved, the solubility problem of the drug can be effectively solved, and the effective loading of the drug and the controlled release of the drug at target sites of tumors can be realized; the dispersity and biocompatibility of magnetic carriers are improved, the long circle, the active targeting and the fixed-point gate-controlled release of the drug are realized; and furthermore, the preparation process is simple, energy saving, environmentally friendly and low in cost, the industrial production can be realized, and the preparation process is the innovation of the tumor treating drugs.