The invention provides a non-aqueous electrolyte secondary battery. A separator 300 includes a base material layer 301 and a heat resistance layer 302. The heat resistance layer 302 includes, in a direction of thickness of the heat resistance layer 302, a central portion 302b and a first end portio 302a and a second end portion 302c between which the central portion lies. The first end portion includes an interface with an electrode. The second end portion 302 includes an interface with the base material layer. A composition ratio of a resin binder to a total mass of inorganic particles and the resin binder in the first end portion and the second end portion is not lower than 8 mass % and not higher than 30 mass %. A composition ratio of the resin binder to a total mass of the inorganic particles and the resin binder in the central portion 302b is not lower than 2 mass % and not higher than 7 mass %.