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发明公开
CN106636150A 一种海洋低温糖原分支酶基因的克隆及其表达 无效 - 驳回

一种海洋低温糖原分支酶基因的克隆及其表达
摘要：
本发明属于酶工程和基因工程领域，具体涉及到一种在低温下仍具有活性与稳定性的海洋低温糖原分支酶基因的克隆与其表达。海洋低温糖原分支酶基因序列为SEQ ID NO.1所示，该基因序列全长1788bp，以ATG为起始密码子，以TGA为终止密码子，GC碱基含量为37％，海洋低温糖原分支酶基因的蛋白序列为SEQ ID NO.2所示，该蛋白是595个氨基酸构成的蛋白序列，预测其分子相对质量为69.97kDa。本发明通过苏云金芽孢杆菌菌株能快速，准确的获得活性最适温度为15‑25℃的低温糖原分支酶基因，并且获得的基因是一个完整的阅读框，并实现快速高效表达低温糖原分支酶基因，可以大量获取海洋低温糖原分支酶，为研究开发低温糖原分支酶提供基础。
摘要（英）：

The invention belongs to the fields of enzyme engineering and gene engineering and particularly relates to cloning and expression of a marine low-temperature glycogen branching enzyme gene with activity and stability even at the low temperature. The sequence of the marine low-temperature glycogen branching enzyme gene is represented as SEQ ID NO.1, overall length of the sequence of the gene is 1788 bp, ATG (antithymocyte globulin) is taken as an initiation codon, TGA (thyroglobulin antibodies) is taken as a termination codon, and content of GC base is 37%; the protein sequence of the marine low-temperature glycogen branching enzyme gene is represented as SEQ ID NO.2, the protein is formed by 595 amino acids, and relative molecular mass of the protein is predicated to be 69.97 kDa. The low-temperature glycogen branching enzyme gene with optimum activity temperature being 15-25 DEG C can be rapidly and accurately obtained from bacillus thuringiensis strains, the obtained gene is a complete reading frame, the low-temperature glycogen branching enzyme gene is rapidly and efficiently expressed, a large amount of marine low-temperature glycogen branching enzyme can be obtained, and a foundation is laid for research and development of the low-temperature glycogen branching enzyme.

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