The invention provides a method for carrying out efficient instantaneous transformation on a woody plant pollen gene gun, and a kit used in the method. The kit comprises the following reagent combination: a reagent for preparing gold powder suspension liquid, a regent for preparing deoxyribonucleic acid (DNA) bullets, and a pollen fluid medium. According to the method, apple pollen is taken as a test material, and the efficient gene gun instantaneous transformation is carried out by preparing a pollen hydrate and the DNA bullets and using helium to bombard pollen grains which are just germinated. After the method is adopted, the functions of a woody plant material pollen tube gene can be rapidly, sensitively, simply and conveniently verified with high accuracy, so that a technical system is provided for visually identifying the gene expression in a woody plant pollen tube and the functional verification of the woody plant pollen tube.