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发明公开
CN106834286A 一步法快速构建扩增子文库的引物组合 有权

一步法快速构建扩增子文库的引物组合
摘要：
本发明公开了一步法快速构建扩增子文库的引物组合，其包括：根据目标扩增子设计的上游融合引物，所述上游融合引物包括第一接头序列和根据目标扩增子设计的特异性上游引物序列；根据目标扩增子设计的下游融合引物，所述下游融合引物包括第二接头序列和根据目标扩增子设计的特异性下游引物序列；上游通用引物，所述上游通用引物包括第三接头序列、barcode序列和第一接头序列；和下游通用引物，所述下游通用引物包括通用序列和第二接头序列。运用该融合引物组合可以简便、快速的经1步构建扩增子文库，且由于在PCR起始前就引入barcode，使得样本及文库间交叉污染的可能性大大降低。
摘要（英）：

The invention discloses a primer combination for rapidly constructing an amplicon library through a one-step process. The primer combination comprises a forward fusion primer designed according to a target amplicon, a reverse fusion primer designed according to the target amplicon, a forward universal primer and a reverse universal primer, wherein the forward fusion primer comprises a first linker sequence and a specific forward primer sequence designed according to the target amplicon; the reverse fusion primer comprises a second linker sequence and a specific reverse primer sequence designed according to the target amplicon; the forward universal primer comprises a third linker sequence, a barcode sequence and the first linker sequence; and the reverse universal primer comprises a universal sequence and the second linker sequence. According to the fusion primer combination, the amplicon library can be simply and rapidly constructed through one step, and before the PCR is started, the barcode is introduced, so the possibility of cross contamination between the samples and the library is greatly reduced.

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