The invention relates to a method for increasing the one-call exception resolution rate of an agent application system. The method comprises the following steps that: (1) service counter service handling personnel answers an agent repair call; (2) the service handling personnel checks data automatically acquired by a system in an agent desktop exception repair tool and a conclusion analyzed automatically by means of database service processing; (3) a judgment on whether an exception type can be determined or not according to the data and the automatically-analyzed conclusion is made; (4) if the exception type cannot be determined, on-site operation and maintenance personnel is assigned to check and handle on an exception site; (5) if the exception type can be determined, the service handling personnel remotely issues an instruction; (6) if the agent cannot understand the instruction, the on-site operation and maintenance personnel is assigned to the exception site; and (7) if the agent can resolve an exception under the remote guidance of the service handling personnel, one-call successful repair resolution is realized. Through adoption of the method, rapid location and accurate description are realized; exceptional site information is kept immediately and comprehensively; and the one-call repair success rate is increased from 41.37 percent to 95.23 percent.