The invention relates to a plateau enzyme fermentation process which specifically comprises the following steps: (1) preparing fresh plateau fruit raw juice; (2) preparing plateau plant raw juice; (3) preparing plateau vegetable raw juice; (4) preparing crushed plateau edible fungi; (5) mixing the four raw materials in steps (1 to 4) and adding poly-sugar alcohol and probiotics for sealed fermentation at the temperature of 28 DEG C for 60 days; (6) putting mixed enzymes after fermentation is finished into a concentrator and sterilizing at the temperature of 120 DEG C for 40 min, so as to obtain a product. By providing the scientific and safe enzyme preparation process, a vegetable enzyme, a herbal enzyme, a cereal enzyme and a beam enzyme are fully combined together, so that nutritional ingredients are more sufficient and more balanced. Therefore, the purposes of being safe and sanitary in use of the enzyme, enhancing the vitality of the enzyme, being beneficial to the body need of people for the enzyme and the absorption of people for the enzyme, promoting body health of people and improving living quality are achieved.