The invention discloses an artificially-created male sterile line for maize and a high-efficient transfer breeding method. By utilizing a gene editing technology, fixed point modification on a maize sterility gene Ms26 is carried out, and a No.5 exon is deleted, so that a protein function of Ms26 gene expression is changed, male sterile plants are created, and a basis is provided for utilization of heterotic vigor of maize. According to the artificially-created male sterile line disclosed by the invention, plants containing gene editing transformants are used as mutant donors and are hybridized with acceptor materials; by oriented mutation in a living body, wild type genes in the acceptor materials can be directly mutated, self-crossing is carried out on second backcross generation to obtain the male sterile material for the maize having an acceptor background; and therefore, the linkage drag of backcross introduction is avoided, the backcross transfer breeding efficiency is also greatly improved, and the breeding period is shortened.