The invention discloses a method of vinegar bioaugmentation with a marine-derived strain of streptomyces capable of producing protease, and belongs to the technical field of food fermentation. A strain of streptomyces (streptomyces sp. CCTCC No: M 2017210) is screened from the marine environment, and the streptomyces is resistant to high temperature and capable of producing protease in high yield. Bacterial powder is prepared from the strain and used for reinforcing acetic fermentation of vinegar. By means of the method, the amino acid content in the vinegar can be improved, and the product flavor and quality of the vinegar can be improved.