The invention discloses a quinoxaline derivative. The structural formula of the quinoxaline derivative is shown as the formula (I) or the formula (II). The invention further discloses a preparation method of the quinoxaline derivative and an application of the quinoxaline derivative in preparation of antitumor drugs. The quinoxaline derivative provided by the invention can interact with intracellular vimentin namely new target points, so that the proliferation and the migration of tumor cells can be restrained, and the quinoxaline derivative can be applied in development of novel antitumor drugs. Besides, the quinoxaline derivative is small in toxicity to normal cells, and when being used for preparing the anticancer drugs, the quinoxaline derivative is high in safety. The quinoxaline derivative has high restraining effect on various tumor cells, and during preparation of the antitumor drugs, the quinoxaline derivative has high medical value and broad market prospects.