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发明公开
CN107522768A 蛋白质脱酰胺化的检测方法及其应用 无效 - 驳回

蛋白质脱酰胺化的检测方法及其应用
摘要：
本发明提出了蛋白质前处理方法、蛋白质脱酰胺化的检测方法及其应用。本发明所提出的蛋白质前处理方法能够充分消除蛋白二级结构的影响，充分暴露蛋白质中氨基酸的结构，进而便于后续对变性酶解后蛋白样品的检测，并且本发明所提出的方法不会引起蛋白质原氨基酸的结构变化，进而能够有效避免对后续检测的干扰。本发明所提出的蛋白质脱酰胺化的检测方法，能够特异、灵敏、快速、准确地检测蛋白质的脱酰胺化程度，可作为蛋白质生物药生产的重要的质控检测手段。
摘要（英）：

The invention provides a protein pretreatment method, a detection method for protein deamidation and an application of the detection method. The protein pretreatment method provided by the invention can fully eliminate the influence of a protein secondary structure, and makes a structure of amino acids in a protein fully exposed, so as to facilitate subsequent detection of a protein sample after denaturation enzymolysis; moreover, the method provided by the invention does not cause changes in the structure of the original amino acids of the protein, and can effectively avoid the interference on subsequent detection. The detection method for protein deamidation can detect the degree of deamination of the protein in a specific, sensitive, rapid and accurate manner, and can be used as an important quality control and detection means for protein biological medicine production.

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