The invention discloses a method for evaluating the bitterness of fructus citrus sarcodactylis juice used for making beer, and belongs to the field of beer quality evaluation. The content of limonin in a sample related to the fructus citrus sarcodactylis juice is compared and simulated with a bitterness value of normal hops, and thus the purpose of conducting standardizing treatment on the fructuscitrus sarcodactylis juice and the bitterness degree of a product thereof is achieved.