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发明公开
CN108753835A 一种利用CRISPR/Cas9编辑猪BMP15基因的方法 无效 - 驳回

一种利用CRISPR/Cas9编辑猪BMP15基因的方法
摘要：
本发明公开了一种利用CRISPR/Cas9编辑猪BMP15基因的方法，包括在目的猪基因组的BMP15基因的外显子1上设计两条gRNA，分别构建至pX458和pX459载体，使BMP15基因发生DNA片段的精确删除而丧失功能。与单条gRNA介导的编辑相比，本发明的方法中外显子DNA片段的精确删除能够更加有效地使BMP15基因丧失功能。
摘要（英）：

The invention discloses a method for editing a swine BMP15 (bone morphogenetic protein 15) gene by using CRISPR/Cas9. The method comprises the steps as follows: designing two gRNAs on an exon 1 of theBMP15 of a target swine genome, and constructing pX458 and pX459 vectors respectively, so that the BMP15 gene loses functions due to accurate deletion of DNA fragments. Compared with single gRNA medicated editing, the method has the advantage that the BMP15 gene can lose functions more effectively due to accurate deletion of DNA fragments of the exon.

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