The invention provides an immobilization method of cephalosporin acylase, and belongs to the technical field of enzyme immobilization. The immobilization method comprises the following steps: (1) a bridging body solution is dropwise added into a carrier solution, a first condensation reaction is conducted for 2-5 h, then first solid-solution separation is conducted, a solid-phase component is collected, and a carrier with a bridging body is obtained; and (2) the carrier, with the bridging body, obtained in the step (1) is mixed with a cephalosporin acylase aqueous solution to be stirred to obtain a mixture solution, triithylamine is dropwise added into the mixture solution, a second condensation reaction is conducted for 20-40 h, then second solid-solution separation is conducted, and a solid-phase component, namely the immobilized cephalosporin acylase is collected, wherein the bridging body is halogen acyl halide with a main chain containing 2-8 carbon atoms, and the carrier is aminoresin. The cephalosporin acylase prepared through the immobilization method is high in enzymatic activity and good in stability.