The invention belongs to the field of biological chemistry, and particularly discloses a magnetic immobilized enzyme vector with a core-shell structure and a preparation method and application of themagnetic immobilized enzyme vector. The preparation method comprises the following steps: adding ferroferric oxide nano particles into a Tris-HCl buffer solution which contains dopamine, stirring for8-24 h at the temperature of 20-30 DEG C, and washing and drying to obtain ferroferric oxide dopamine core particles; dissolving the ferroferric oxide dopamine core particles, zirconium chloride and lauric acid in an organic solvent, carrying out ultrasonic treatment, then adding amino terephthalic acid, insulating for 1-3 h at the temperature of 75-85 DEG C, then heating to the temperature of 100-120 DEG C and insulating for 10-24 h, and carrying out aftertreatment to obtain the final product. The obtained magnetic immobilized enzyme vector is easy to separate, can be reused, and is high in enzyme load; and the prepared magnetic immobilized enzyme vector is applied to immobilization of amidase, and thus, the obtained immobilized amidase is high in enzyme activity recycling rate and good in thermal stability and organic solvent tolerance.