The invention relates to probiotics powder for regulating constipation and a preparation method thereof, and belongs to the technical field of food processing and production. Raw materials include a plurality of beneficial substances of lactobacillus rhamnosuslyophilized powder, bifidobacterium longum lyophilized powder, lactobacillus plantarum lyophilized powder, lactobacillus acidophilus lyophilized powder, bifidobacterium breve lyophilized powder, bifidobacterium lactis lyophilized powder, sorbitol, resistant dextrin, lactitol, oligofructose, stachyose and the like. At the same time, the preparation process is improved, and the raw materials are separately divided into several parts to prepare mixed powder and then further mixed with other raw materials. An obtained solid beverage not only has a certain conditioning effect on the constipation, but also has excellent product properties, and is not prone to moisturize and agglomerate. The solubility, uniformity and full powder yield are high.