The invention provides a high performance liquid chromatography detection method of a cangrelor related substance. The method comprises the following steps: adopting a reversed-phase chromatographic column; the filler being octadecyl bonded silica gel; and carrying out gradient elution by taking a phosphate sodium perchlorate buffer solution as a mobile phase A and acetonitrile as a mobile phase B. The method has the advantages of strong specificity, high sensitivity and accurate quantitative determination result; the analysis method is simple, convenient and rapid, can better separate all impurities in a short time, has a separation degree between each chromatographic peak of more than 1.5, can comprehensively detect the cangrelor related substance and the cangrelor related substance in the preparation of the cangrelor, improves the safety of the medicine, and provides a basis for formulating the quality standards of the cangrelor bulk drug and the preparation of the cangrelor.