The invention relates to the technical field of medicine chemistry, in particular to a heteroaromatic ring compound with a thiosemicarbazone structure unit and a preparation method and application ofthe compound. The heteroaromatic ring compound with the thiosemicarbazone structure unit is chelated with metal ions in gastric cancer cells to form a stable complex, and accordingly the proliferationactivity of the gastric cancer cells MGC803 is inhibited. It is proved through a result of an embodiment that compared with a compound 3-AP, the heteroaromatic ring compound with the thiosemicarbazone structure unit has good proliferation inhibition activity on the gastric cancer cells MGC803.