The invention discloses a fluff removing system for train air conditioner filtering screen cleaning. The fluff removing system comprises a support frame, a conveying mesh belt, a lifting frame, a hairbrush roller pair, a lifting mechanism and a cleaning groove, wherein the conveying mesh belt is installed on the support frame, the hairbrush roller pair comprises two hairbrush rollers vertically arranged opposite to each other, a gap is arranged between the two hairbrush rollers, one hairbrush roller is arranged on the support frame, and is driven to rotate by a first motor, the other hairbrushrollers is arranged on the lifting frame, and is driven to rotate by a second motor, the lifting mechanism comprises a third motor, a gear and a gear rack, the third motor, the gear and the gear rackare arranged on the support frame, and the cleaning groove is arranged on the support frame, and is positioned below the hairbrush rollers. According to the present invention, fluff is removed by therotation of the hairbrush roller pair, such that the removing effect is good, the train air conditioner filtering screen is easily subjected to water washing after fluff removing, the meshes of the train air conditioner filtering screen are no longer blocked, and the air conditioner operation effect is good.