The invention relates to a method for extracting and separating sesquiterpene lactone compounds from artemisia sacrorum ledeb. The sesquiterpene lactone compounds comprise 1alpha-acetoxyeudesm-4-en-6beta,11betaH-12,6-olide; (11S)-3-oxoeudesma-1,4(15)-dieno-12,6 alpha-lactone; 1-epi-dehydroisoerivanin; 11-epitaurin; chrysanthemolide; 1 alpha-acetyl-gallicadiol; erivanin; 1 alpha,4alpha-dihydroxyeudesm-2-en-5alpha,6beta,11betaH-12,6-olide; vulgarin; (+)-dehydrovomifoliol. The extracting and separating method comprises the steps that by adopting silica gel, an ODS open column and a semi-preparative liquid chromatography and other chromatographic methods, an ethyl acetate extraction part of a 95% ethyl alcohol extract of the artemisia sacrorum ledeb is separated and purified, and the above 10compounds are obtained through separation. The extraction method is simple, the extraction efficiency is high, the number of the extracted compounds is large, and a foundation is laid for the pharmacological study of the sesquiterpene lactone compounds.