The invention discloses a method for evaluating utilization capacity of intestinal florae for alginate. The alginate is dietary fiber often eaten by people and can be utilized by microorganisms in intestinal tracts to influence health of organisms. By analysis of the correlation between sequencing results of intestinal florae of multiple samples and the utilization capacity of the intestinal florae for the alginate, 7 intestinal microbial genera in significant correlation with the utilization capacity for the alginate are provided. The method provided by the invention evaluates the utilizationcapacity of the intestinal florae for the alginate by score calculation by use of high-throughput sequencing results of excrement florae. The method needs simple data analysis only, is convenient andrapid, requires no actual in-vivo or in-vitro fermentation experiments and saves time, labor and experiment costs.